Wide Pore Protein Columns

InsulinThe rapid progress in the areas of genomics, proteomics, metabolomics and other biotechnology sectors has pushed scientists to develop innovative and efficient chromatographic methods. These methods have opened the way to better understanding of biomolecules and now offer impactful solutions effective at each level of the development of new commercial biopharmaceutical ingredients. Sorbent materials used in biochromatography and small molecule chromatography are similar but they require specific characteristics such as wide pore sizes and/or precise chemical resistance. Separation and determination of peptides, proteins and nucleic acids can be done via different chromatography techniques. This section will highlight the HPLC columns used in each following technique:

  • Reversed-phase biochromatography for MW between 5,000 and 100,000 Da.
  • Ion exchange chromatography (IEC)
  • Size exclusion chromatography (SEC)

 

Reversed-phase biochromatography for MW between 5,000 and 100,000 Da.

  • Choose Fortis Bio C18 and C4 for UPLC and Analytical Separations
  • Choose COSMOSIL Protein-R for high recovery rate and acid-resistance
  • Choose SiliaChrom XDB1 for prep scale separations

 

Column Phase  Pore Size (Å) Particle Sizes (µm) Surface Area (m2/g)

Carbon Load (%)

 COSMOSIL Protein-R C18 300 5 150 -
 COSMOSIL C18-AR-300 C18 300 5 150 12
 Fortis Bio C18 C18  300 1.7, 5  -
 SiliaChrom XDB1 C18 C18 300 5, 10 80 8
 COSMOSIL C8-AR-300 C8 300 5 150 7
 SiliaChrom XDB1 C8 C8 300 5 80 4
 COSMOSIL C4-AR-300 C4 300 5 150 6
 Fortis Bio C4 C4 300 1.7, 5  -
 SiliaChrom XDB1 C4 C4 300 3, 5, 10 80 3
 SiliaChrom XDB1 CN Cyano 300 5 80 3.5
 COSMOSIL Ph-AR-300 Phenyl 300 5 150 7
 SiliaChrom XDB1 Phenyl Phenyl 300 5 80 4.5

 

 

Ion Exchange

 

 

Column Phase Pore Size (Å) Particle Sizes (µm) Surface Area (m2/g)

Carbon Load (%)

COSMOSIL Diol 120-II Diol 120 5 - -
COSMOSIL Diol 300-II Diol 300 5 - -
SiliaChrom IEC SAX-300 SAX 300 3, 5 80
SiliaChrom IEC SCX-300 SCX 300 3, 5 80 3.5