SiliaChrom SB HPLC Columns

The SiliaChrom SB C8 and C18 are specially designed for the separation of polar and mid-polar compounds from low to medium pH. A class of columns offering excellent performance for LC-MS applications. The surface of SiliaChrom SB C8 and C18 are treated with an organic form of silicon to increase the number of silanol groups on the surface. After this, the surface is bonded with an octadecylsilane with two different fonctions. One of them is a protecting group that shields the area under it and protects the surface from an acid attack from the mobile phase.

• Extremely low pH Limits (0,5-7,5)
• Extremely low bleed for LC-MS applications under acidic conditions
• Compatible with mobile phases ranging 100% aqueous to 100% organic
• Non-endcapped

Column Performance Evaluation - Ethylbenzene

• Chromatographic conditions
• Column: SiliaChrom SB C18, 5 μm
• Column size: 4.6 x 150 mm SiliCycle P/N: H101805H-N150
• Mobile phase: 2% TFA in ACN/water (60/40) Solution pH: 1.00
• Temperature: 23°C
• Flow rate: 1.000 mL/min
• Detector: UV at 270 nm
• Injection volume: 10 μL

Ordering Information